說明
The records in this dataset were collected and evaluated for the following publication: Porco, D., Hermant, S., Purnomo, C.A. et al. eDNA-based detection of the invasive crayfish Pacifastacus leniusculus in streams with a LAMP assay using dependent replicates to gain higher sensitivity. Sci Rep 12, 6553 (2022). https://doi.org/10.1038/s41598-022-10545-w
Abstract of the publication: LAMP assays are becoming increasingly popular in the feld of invasive species detection but are still underused in eDNA-based monitoring. Here, we propose a LAMP assay designed to detect the North American crayfsh species Pacifastacus leniusculus in water samples from streams. The presence of P. leniusculus was detected through this new LAMP assay in all but one of the nine sites sampled. No correlation was found between ddPCR absolute concentration measurements and the number of LAMP-positive technical replicates. However, we showed that using dependent technical replicates could signifcantly enhance the detection sensitivity of the LAMP assay. Applied to other assays, it could improve sensitivity and thus allow for a more efcient use of eDNA-based LAMP assays for invasive species detection in aquatic ecosystems.
資料紀錄
此資源出現紀錄的資料已發佈為達爾文核心集檔案（DwC-A），其以一或多組資料表構成分享生物多樣性資料的標準格式。 核心資料表包含 340 筆紀錄。
此 IPT 存放資料以提供資料儲存庫服務。資料與資源的詮釋資料可由「下載」單元下載。「版本」表格列出此資源的其它公開版本，以便利追蹤其隨時間的變更。
版本
以下的表格只顯示可公開存取資源的已發布版本。
如何引用
研究者應依照以下指示引用此資源。:
Porco D (2022): eDNA‑based detection of the invasive crayfish Pacifastacus leniusculus in streams with a LAMP assay using dependent replicates to gain higher sensitivity. v1.10. National Museum of Natural History, Luxembourg. Dataset/Occurrence. https://gbif.mnhn.lu/ipt/resource?r=edna_pacifastacus_leniusculus_2022&v=1.10
權利
研究者應尊重以下權利聲明。:
此資料的發布者及權利單位為 National Museum of Natural History, Luxembourg。 To the extent possible under law, the publisher has waived all rights to these data and has dedicated them to the Public Domain (CC0 1.0). Users may copy, modify, distribute and use the work, including for commercial purposes, without restriction.
GBIF 註冊
此資源已向GBIF註冊，並指定以下之GBIF UUID: ea16e238-4e23-41fb-9eee-c1ea4f0caa63。 National Museum of Natural History, Luxembourg 發佈此資源，並經由GBIF Luxembourg同意向GBIF註冊成為資料發佈者。
關鍵字
Occurrence
聯絡資訊
- 出處 ●
- 連絡人
https://orcid.org/0000-0003-1914-1973
- 元數據提供者 ●
- 連絡人
地理涵蓋範圍
Luxembourg
| 界定座標範圍 | 緯度南界 經度西界 [49.425, 5.691], 緯度北界 經度東界 [50.198, 6.537] |
|---|
時間涵蓋範圍
| 起始日期 / 結束日期 | 2021-10-07 / 2021-10-11 |
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計畫資料
DNADIVE aims at developing a toolbox for the molecular monitoring of invasive crayfish in the streams of Luxembourg. Three exotic species (Orconectes limosus, Pacifastacus leniusculus and Astacus leptodactylus) and a native one (Astacus astacus) will be targeted for the project. This molecular toolbox will encompass several techniques of detection comprising (1) a simple amplification method easily performed in a laboratory with few elements, (2) a digital droplet amplification (ddPCR) which is a more elaborated lab method that can allow for a higher detection sensitivity and a possible quantification of DNA that could be related through the proxy of biomass and abundance to the size of the populations detected and (3) an isotherm amplification method (iPCR) i.e. a simple, cost effective approach which will allow for a field detection usable by non-trained agents. The results will enable the development of a predictive species distribution model for the target species and to infer their impact on freshwater communities through the comparison with previous sampling campaigns.This set of methods has the high potential to efficiently contribute to early detection and routine monitoring of the invasive crayfish species in Luxembourg, thus allowing for a timely and efficient decision-making and appropriate management.
| 計畫名稱 | eDNA Detection for crayfish InvasiVE species in streams of Luxembourg: development of a molecular toolbox |
|---|---|
| 辨識碼 | fnr_6a3662d14403588d48664fb09ec37a0b |
| 經費來源 | National Research Fund Luxembourg 2, Avenue de l'Universite (Maison du Savoir B.P. 1777) L-1017 Luxembourg, 4365 Esch-sur-Alzette Grant Number: fnr_6a3662d14403588d48664fb09ec37a0b |
參與計畫的人員:
額外的詮釋資料
| 替代的識別碼 | ea16e238-4e23-41fb-9eee-c1ea4f0caa63 |
|---|---|
| https://gbif.mnhn.lu/ipt/resource?r=edna_pacifastacus_leniusculus_2022 |