Descrição
The records in this dataset were collected and evaluated for the following publication: Porco, D., Hermant, S., Purnomo, C.A. et al. eDNA-based detection of the invasive crayfish Pacifastacus leniusculus in streams with a LAMP assay using dependent replicates to gain higher sensitivity. Sci Rep 12, 6553 (2022). https://doi.org/10.1038/s41598-022-10545-w
Abstract of the publication: LAMP assays are becoming increasingly popular in the feld of invasive species detection but are still underused in eDNA-based monitoring. Here, we propose a LAMP assay designed to detect the North American crayfsh species Pacifastacus leniusculus in water samples from streams. The presence of P. leniusculus was detected through this new LAMP assay in all but one of the nine sites sampled. No correlation was found between ddPCR absolute concentration measurements and the number of LAMP-positive technical replicates. However, we showed that using dependent technical replicates could signifcantly enhance the detection sensitivity of the LAMP assay. Applied to other assays, it could improve sensitivity and thus allow for a more efcient use of eDNA-based LAMP assays for invasive species detection in aquatic ecosystems.
Registros de Dados
Os dados deste recurso de ocorrência foram publicados como um Darwin Core Archive (DwC-A), que é o formato padronizado para compartilhamento de dados de biodiversidade como um conjunto de uma ou mais tabelas de dados. A tabela de dados do núcleo contém 340 registros.
This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.
Versões
A tabela abaixo mostra apenas versões de recursos que são publicamente acessíveis.
Como citar
Pesquisadores deveriam citar esta obra da seguinte maneira:
Porco D (2022): eDNA‑based detection of the invasive crayfish Pacifastacus leniusculus in streams with a LAMP assay using dependent replicates to gain higher sensitivity. v1.10. National Museum of Natural History, Luxembourg. Dataset/Occurrence. https://gbif.mnhn.lu/ipt/resource?r=edna_pacifastacus_leniusculus_2022&v=1.10
Direitos
Pesquisadores devem respeitar a seguinte declaração de direitos:
O editor e o detentor dos direitos deste trabalho é National Museum of Natural History, Luxembourg. To the extent possible under law, the publisher has waived all rights to these data and has dedicated them to the Public Domain (CC0 1.0). Users may copy, modify, distribute and use the work, including for commercial purposes, without restriction.
GBIF Registration
Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: ea16e238-4e23-41fb-9eee-c1ea4f0caa63. National Museum of Natural History, Luxembourg publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por GBIF Luxembourg.
Palavras-chave
Occurrence
Contatos
- Originador ●
- Ponto De Contato
https://orcid.org/0000-0003-1914-1973
- Provedor Dos Metadados ●
- Ponto De Contato
Cobertura Geográfica
Luxembourg
| Coordenadas delimitadoras | Sul Oeste [49,425, 5,691], Norte Leste [50,198, 6,537] |
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Cobertura Temporal
| Data Inicial / Data final | 2021-10-07 / 2021-10-11 |
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Dados Sobre o Projeto
DNADIVE aims at developing a toolbox for the molecular monitoring of invasive crayfish in the streams of Luxembourg. Three exotic species (Orconectes limosus, Pacifastacus leniusculus and Astacus leptodactylus) and a native one (Astacus astacus) will be targeted for the project. This molecular toolbox will encompass several techniques of detection comprising (1) a simple amplification method easily performed in a laboratory with few elements, (2) a digital droplet amplification (ddPCR) which is a more elaborated lab method that can allow for a higher detection sensitivity and a possible quantification of DNA that could be related through the proxy of biomass and abundance to the size of the populations detected and (3) an isotherm amplification method (iPCR) i.e. a simple, cost effective approach which will allow for a field detection usable by non-trained agents. The results will enable the development of a predictive species distribution model for the target species and to infer their impact on freshwater communities through the comparison with previous sampling campaigns.This set of methods has the high potential to efficiently contribute to early detection and routine monitoring of the invasive crayfish species in Luxembourg, thus allowing for a timely and efficient decision-making and appropriate management.
| Título | eDNA Detection for crayfish InvasiVE species in streams of Luxembourg: development of a molecular toolbox |
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| Identificador | fnr_6a3662d14403588d48664fb09ec37a0b |
| Financiamento | National Research Fund Luxembourg 2, Avenue de l'Universite (Maison du Savoir B.P. 1777) L-1017 Luxembourg, 4365 Esch-sur-Alzette Grant Number: fnr_6a3662d14403588d48664fb09ec37a0b |
O pessoal envolvido no projeto:
Metadados Adicionais
| Identificadores alternativos | ea16e238-4e23-41fb-9eee-c1ea4f0caa63 |
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| https://gbif.mnhn.lu/ipt/resource?r=edna_pacifastacus_leniusculus_2022 |